Histology and Histochemistry Laboratory

- Services  -

The following dyeing techniques are applied in tissue sections:

• Hematoxylin & eosin (tissues in general)
• Masson's trichrome (muscle and collagen)
• Mallory's trichrome (conjunctive tissue)
• PAS Alcian blue (acid and neutral mucopolysaccharides)
• Peryodic Acid-Schiff PAS (glucogen, mucins)
• Feulgen (DNA, collagen and conjunctive tissue)
• Toluidine blue (mucins)
• Oily red (fat)
• Sudan IV (neutral fat)
• Sudan black (triglycerides, phospholipids, cholesterol esters)
• Ninhydrin-Schiff (proteins, amino groups)

The laboratory has the following equipment:

• Leica RM 2025 and RM 2155 rotation microtomes for paraffin and resin sections
  
• MICROM HM 505N cryostat for freezing and cutting frozen sections
• Millipore incubator
  
• VWR Scientific incubator
  
• COLE PARMER oven
  
• OHAUS scale
• 2 HISTOEMBEDDER Leica paraffin embedding systems.
• Olympus BX41 Microscope for bright field and phase contrast
  
• Olympus BX51 Fluorescence microscope
  
• IMAGE-PRO PLUS Olympus-Media Cybernetics Image analysis system
  

The laboratory offers technical support for setting up new histochemical and immune histochemical techniques where infrastructure possibilities are taken into account.

Conditions for delivering samples:

• Tissue samples must be adequately fixed or preserved according to the recommended protocol for the technique applied for and delivered to the laboratory duly labeled.
• The time needed to attend a service is in function of the number of samples. It is necessary to agree previously on a delivery date with the Responsible technician in charge of the laboratory for an adequate programming.

Sample reception is from Monday through Friday from 8 to 15:00 h.
For more information please contact the technician in charge of the laboratory or write to: This e-mail address is being protected from spambots. You need JavaScript enabled to view it