Marine Dinoflagellate Collection
There are two methodological strategies to obtain strains: (a) induction to
cyst germination according to the biological method of cleaning and
concentrating cysts, and (b) isolation of vegetative forms with micropipette,
starting from phytoplankton samples collected with a net or concentrated with
The strains are maintained in liquid, using f/2 and GSe culture mediums. Culture room
conditions are maintained at 20 ± 2 and 25 ± 2 °C; cycles
of light-darkness of 12:12 h; and 2,200 Lux of average luminous intensity.
Re-inoculation of strains is performed every 25 days.
To identify strains we apply traditional
light microscopy methods. The dinoflagellates are identified with theca dissection
technique and the use of stains, while naked dinoflagellates are observed live to
facilitate the location of key morphologic features that allow discriminating
among species. To identify complex species, and those with features that are
difficult to observe with conventional microscopes, a Scanning Electron
Microscope is used.
An iconography record is being compiled with a system of image analysis.
Written by Dra. Lourdes Morquecho Escamilla
Last Updated on Thursday, 17 June 2010 09:45